spliced overlap extension
方法逆转录-聚合酶链反响分别获得目白勺基因GM-CSF和BZLF1编码序列白勺cDNA,采用剪接式重叠延伸(spliced overlap extension, SOE)技术将两段基因通过多肽接头(Gly4Ser) 3白勺DNA编码序列停止连接,构建交融基因BZGM。
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spliced overlap extension
拼接重叠延伸
以上为机器翻译结果，长、整句建议使用 人工翻译 。
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Methods: Genes were spliced by overlap extension.
方法：采用重叠区扩增基因拼接法。
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METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
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