Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.

目的：评价异源双链分析（HET）和单链构象多态性分析（SSCP）在基因突变检测中的作用。

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Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.

用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。

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