However, only a single copy of the gene is mutated in tumours, raising the possibility that the mutations do not result in a simple loss of function.

然而，在肿瘤中只有一个基因的单一复制出现突变，那就会增加突变的可能性而不仅仅是导致一个简单功能的丢失。

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Transgenes should be with single copy and without accessory sequence, and with consistence to express and stable genetic in various transgenic plants in a new gene transformation program.

新的转化程序要求把基因导入农艺性状优良的品种中，呈单拷贝、不带有辅助序列，并在不同的转化体中表达一致，稳定遗传。

youdao

Transgenes should be genes with single copy and without marker gene, and be able to express and stable inheritable in various transgenic plants in a new gene transformation program.

新的基因转化程序要求转基因为单拷贝，不带有标记基因，并在不同的转化体中表达一致，稳定遗传。

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