Methods a modified telomeric repeat amplification protocal (TRAP) by silver staining was performed in 19 cases of intracranial tumors and 5 cases of brain injuries.
方法取19例颅内肿瘤和5例脑外伤患者脑脊液标本,应用改良的银染端粒重复序列扩增法(TRAP)进行端粒酶活性检测。
Methods Telomerase activity was examined in 37 brain tumor tissues and 4 normal human brain tissues by telomeric repeat amplification protocol (TRAP) assay and silver staining.
方法采用TRAP -银染法检测了37例脑肿瘤组织和4例正常脑组织中的端粒酶活性。
Methods The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining.
方法DNA样本来自广东汉族男性无关个体,PCR后用聚丙烯酰胺凝胶电泳和银染法进行DNA分型。
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