semi-nested RT-realtime PCR 半巢式反转录实时荧光PCR
Furthermore, with semi-nested RT-PCR approach, we also characterized 13 ClassⅠtype NDV strains, which were rarely characterized by the conventional methods.
而且,传统方法很难区分的13株Class I毒株,使用此半巢式RT-PCR方法亦成功区分。
参考来源 - 新城疫强弱毒半巢式RT·2,447,543篇论文数据，部分数据来源于NoteExpress
So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.
因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
Methods:HGV RNA was detected by a semi nested RT PCR.
方法：通过半巢式逆转录多聚酶链反应检测HGV。
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