Objective To evaluate the reliability of colony PCR in identifying the recombinant clones selected from phage antibody libraries.
目的评价菌落pcr法鉴定噬菌体抗体库阳性重组率的可靠性。
Methods: Using the influenza A3 split vaccine as the target molecule, peptides that could bind with target molecule were selected from the phage display 12 peptide library by 3 rounds of biopaning.
方法：以甲三型流感病毒裂解疫苗原液为靶分子，经过3轮生物淘选，从噬菌体随机肽库中筛选与之结合的噬菌体。
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