Conclusion: Recombinant T7 phage vaccine expressing xenogenic VEGF can break immunologic tolerance against self-VEGF and induce the producing of specific anti-VEGF antibody.

结论：异种血管内皮生长因子基因重组T7噬菌体疫苗可打破机体对自身vegf的免疫耐受，诱导产生较高水平的特异性抗vegf抗体。

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Objective To evaluate the reliability of colony PCR in identifying the recombinant clones selected from phage antibody libraries.

目的评价菌落pcr法鉴定噬菌体抗体库阳性重组率的可靠性。

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