Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
结果：荧光定量方法可以快速完成MTHFR C677T基因多态性的检测，检测结果得到传统PCR-RFLP方法的证实。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35s promoter heterologous template for the detection of genetically modified maize was developed in this study.
建立了玉米转基因成分中35s启动子非同源模板的竞争定量pcr检测系统。
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