...斛信息网 0.05mg/l 6-BA+(0.5~2.0)mg/L2.4-D培养基上．由叶基部诱导产生愈伤组织，进而分化出拟原球茎（ Protocorm like bodies， PLBs）获得完整植株，并指出2.4-D对诱导愈伤组织形成和分化有重要作用，NAA的加入对分化PLBs有一定的..
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Objective: To study the relationship between growth of Protocorm-like bodies and accumulation of total alkaloids and polysaccharides (two active constituents) in Dendrobium moniliforme.
目的：研究细茎石斛拟原球茎生长规律及其与两种有效成分总生物碱和可溶性多糖积累的关系。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
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