Purpose Molecular cloning and expression of pro nattokinase gene with e.
目的构建纳豆激酶原基因克隆，实现其在大肠杆菌中的表达。
Methods Chromosome DNA was isolated from Bacillus natto . The pro nattokinase gene was then amplified from chromosome by PCR.
方法从纳豆芽胞杆菌中分离纯化基因组DNA ，作为模板通过PCR扩增纳豆激酶原基因。
According to reports, Nattokinase has plasmin-like bio-characteristic that lyses fibrin directly, or increases activity of pro-urokinase and t-PA (Tissue Plasminogen Activator).
据报道，纳豆激酶有和胞浆素一样的可直接分解纤维蛋白的生物特性，或者增强尿激酶原和t—PA(人组织型纤溶酶原激活剂)的活性。
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