...词: 日本血吸虫; cDNA文库; 步移法; 生物信息学 [gap=1212]Key words :Schistosoma japonicum; cDNA library ; Primer - walking ; Bioinformatics ...
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Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.
单引物pcr是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。
Conclusion It is a feasible method to discover and acquire novel genes of S. japonicum by ESTs, combination of primer-walking sequncing and bioinformatics technique.
结论表达序列标签法、步移法测序和生物信息学技术有利于发现日本血吸虫新基因。
Methods The whole intron 51 was sequenced by primer walking.
方法用步移法测定第5 1内含子全序列。
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