Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法，从而提高STR分型检验的灵敏度。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
The chloroform solution of 4-Amino-antipyrine was used to measure the phenol in water by extraction and photometer. The method and the precision were discussed in this paper.
本文通过实验的方法，用三氯甲烷处理4- 氨基安替比林溶液，解决了用4-氨基安替比林测定挥发酚实验空白值高的问题，并确定了三氯甲烷最佳使用量。
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