Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法，从而提高STR分型检验的灵敏度。
Objective: Study on extraction of plasmid DNA with phenol-chloroform from clone.
目的：对用酚-氯仿直接从克隆中提取质粒dna的方法进行了研究。
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