Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
A simple and rapid method for screening positive clones by PCR was also established in this paper.
并建立了快速、简便的PCR直接筛选和鉴定阳性克隆的方法。
Conclusion 3bs-pcr, a simple and economical method, could support us with an alternatively valuable assay for screening DNA point mutations from large number samples.
结论3BS - PCR方法操作简单经济，提供了有应用价值的大规模筛检dna点突变的方法。
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