Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
In this article, we reviewed the quantitative method and research progress of digital PCR technology in the main application fields.
本文介绍数字P CR技术的定量方法，并评述该技术在主要应用领域的研究进展。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
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