需具有温度梯度(PCR Gradient)功能，可经由一次实验即可找到最佳条件； 且设定范围需达40-99℃(或更佳)，反应槽需可提供12 个相同温度及 8 个不同 温度梯度(Pr...
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Method the main influential factors were detected by PCR in serial concentration gradient and electrophoresis, then select the optimal experimental conditions.
方法对实验研究中的主要影响因素设置各系列浓度梯度，进行PCR反应后观察电泳结果选取最适条件。
Methods: Gradient PCR was employed in analysis of aldosterone synthase (CYP11B2) gene polymorphisms, and gel Imaging Technology was used to determine the optimal annealing temperature.
方法：采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增，通过凝胶呈像技术判断CYP11 B2基因最佳退火温度。
Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.
结果：与普通pcr相比，梯度P CR能够迅速地确定CYP11 B2基因的最佳退火温度。
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