Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
方法：聚合酶链反应（PCR）、单链构象多态性（SSCP）分析。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Methods The polymorphism if exon 17 and 18 of insulin gene were detected in 46 hypertension patients and 39 normal controls using PCR and single strand conformation polymorphism.
方法采用聚合酶链式反应-单链构象多态性方法检测46例高血压患者和39例正常血压对照者的胰岛素受体基因第17、18外显子的多态性。
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