越来越多的证据表明 E- cadherin基因为抑癌基因 [1~ 5]。我们采用银染聚合酶链反应 -单链构象多肽性分析法 (PCR- SSCP)对 2 5例胃癌患者的 DNA进行了 E- cadherin基因第 8外显子检测 ,现报告如下。1资料与方法1 .
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PCR-SSCP 分析 ; 聚合酶链反应 ; 技术 ; 多态性分析
pcr-sscp分析 pcr-sscp analysis ; PCR-SSCP
RT PCR SSCP 多态性分析
asymmetric PCR-SSCP 不对称PCR
multiplex pcr-sscp 复合pcr
复合PCR-SSCP Multiplex PCR-SSCP
Semi-nested LP-PCR-SSCP 半巢式LP
PCR SSCP 聚合酶链反应单链构像多态性
MF PCR SSCP 多荧光标记PCR单链构象多态性分析
The results of gene array corresponded with that of PCR-SSCP and DNA sequencing.
基因阵列检测结果与PCR SSCP及测序结果一致。
By PCR-SSCP analysis, 17 PCR products were identified with different mobility of single strand DNA in propositus. 9 suspectable mutations were revealed with DNA sequencing analysis.
SSCP分析发现17个外显子pcr产物单链dna迁移率异常,通过DNA直接测序发现9个外显子可疑突变,但反向测序均未能证实。
The polymorphism of the insulin-like growth factor binding protein 3 (IGFBP3) gene in Nanyang cattle, Luxi cattle and Chinese Simmental was analyzed by PCR-SSCP.
采用PCR-SSCP技术分析了牛类胰岛素生长因子结合蛋白3(IGFBP3)基因在南阳牛、鲁西牛和中国西门塔尔牛3个牛品种中的遗传多态性。
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