Methods An ischemic model was developed in PC12 cell line with treatment of oxygen glucose deprivation (OGD).
方法采用氧葡萄糖剥夺(ogd)方法在PC 12细胞上建立缺血模型。
NECL2 distributed in cell body and protuberance of primary culture neuron. The rat adrenal gland tumor cell line PC12 can be induced into neuronal differentiation.
大鼠肾上腺嗜铬细胞瘤细胞PC12具有神经元样分化的潜能，在PC12细胞中转染并过量表达NECL2能够诱导细胞发生神经元样分化。
METHODS Natural products were collected to screen in the stable cell line of GLP-1 receptor, and the active component was identified in the PC12 cells.
方法建立稳定的GLP-1受体激动剂高通量筛选模型，从天然药物中筛选活性成分； 在PC12细胞中验证GLP-1受体激动剂的功能及作用机制。
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