论文:浅析如何改进商誉会计-中大网校论文网 关键词】 抗原 CD59 点突变 真核表达 [gap=1053]KEY WORDS］ Antigens, CD59; Point mutation; Overlap extension PCR; Eukaryotic expression
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中国最大的综合性文献数据库 -维普资讯 关键词: 重叠延伸PCR 猪β防御素2 猪γ干扰素 融合蛋白 毕赤酵母[gap=1444]Key words: overlap extension PCR; PBD-2; PoIFNγ; fusion protein; Pichia pastoris
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splicing by overlap extension PCR 采用重叠延伸PCR ; 重叠延伸PCR
overlap extension pcr technique 重叠延伸pcr法
overlap extension pcr oe pcr 重叠延伸pcr
spliced overlap extension pcr technology 重叠延伸pcr技术
overlap extension of PCR 套叠PCR
PCR-driven Overlap Extension 重叠延伸PCR法
PCR splicing overlap extension 重叠伸展的PCR缝接
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
The common overlap extension PCR was improved and the new methods named as one-step overlap extension PCR, which can reduce the PCR times and improve the efficiency.
对常规重叠延伸P CR技术进行了改进，改进后的方法称之为一步法重叠延伸pcr，减少了进型PCR反应的次数，提高了效率。
Conclusion Splicing overlap extension by PCR is a convenient method to obtain recombinant gene.
结论：重叠延伸pcr是一种获得重构基因的简捷方法。
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