Abstract Objective To improve the diagnosing level of tuberculosis meningitis (TBM) by means of modified preparation of cerebrospinal fluid (CSF) and optimized IS6110 PCR system.
目的 改良脑脊液样本处理方法和优化PCR检测系统，提高结核性脑膜炎诊断阳性率。
Furthermore, the reaction system and program of ERIC-PCR method for the acid mineral drainage were optimized.
确定了ERIC方法用于酸性矿坑水样品时的最佳扩增体系和反应程序。
SSR technique was optimized from DNA extraction, PCR procedure and electrophoresis detection. A standard SSR system fitting for maize hybrid identification was established finally.
本研究从DNA提取、PCR扩增、电泳检测等环节对SSR技术进行了优化，最终建立一套完善的适用于玉米品种鉴定的SSR标准体系。
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