原发性肝癌患者外周血GPC-3 mRNA的表达与微转移的关系柏凯-临床医学论文-论文联盟 关键词】 肝细胞癌;GPC-3;巢式RT-PCR;微转移 [gap=881]Key words】HCC; GPC-3; nested RT-PCR; micro-metastasis
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【摘要】： 正目的建立套式逆转录聚合酶链反应(nested RT-PCR)检测临床样本鼻病毒(human rhinovirus, HRV)基因的方法和直接免疫荧光(DFA)的方法对常见呼吸道病毒病原进行检测,了解上海地区小儿...
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方法:采用巢式逆转录聚合酶链反应(nested reverse transcriptase polymerase chain reaction,nested RT-PCR)的方法检测MUC7mRNA在68例膀胱癌患者,15例泌尿系良性疾病患者,以及10例健康人尿脱落细胞中...
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semi-nested rt-pcr 半巢式rt ; 半套式rt
nested-rt-pcr 巢式rtpcr法 ; 聚合酶链反应
immuno-capture nested rt-pcr 免疫捕获巢式rt
MULTIPLEX NESTED RT-PCR 建立一种高度灵敏的能够在单细胞水平检测时钟基因表达的反转录巢式多重聚合酶链式反应
Nested RT PCR 巢式反转录聚合酶链反应
nested rt pcr n pcr 逆转录套式聚合酶链反应
Single cell nested RT-PCR 单细胞巢式RT
multiplex nested-RT-PCR assay 多重巢式反转录聚合酶链反应
RT-nested PCR 式聚合酶链反应 ; 聚合酶链式反应 ; 逆转录套式pcr
Through blood screening for HCV antibodies, the infection rate of HCV in pregnant women in south of China is 1.33% (20/1506). Using nested RT-PCR, 80% (16/20) of the anti-HCV positive pregnant women are HCV RNA positive.
通过抗-HCV进行筛查，我国南方地区孕妇人群抗-HCV阳性率为1.33％(20／1506)，这些抗—HCV阳性的孕妇采用逆转录巢式PCR方法检测HCV RNA，发现80％(16／20)呈HCV RNA阳性。
参考来源 - 丙型肝炎病毒的垂直传播研究No cross-reactions were found in samples of infectious bovine rhinotracheitis virus,bovine influenza virus type 3,and bovine viral diarrhea virus. These results indicated that the nested RT-PCR was specific for BRSV detection.
用该套式RT-PCR对牛传染性鼻气管炎病毒、牛副流感病毒3型、牛病毒性腹泻病毒等进行了检测,结果无交叉反应,表明该检测方法具有良好的特异性。
参考来源 - 套式RTEvaluation of Four BTV detection systems to BTV-5The nucleic acid real-time fluorescent RT-PCR detection system, nucleic acid RT-PCR detection system, OIE standard nested RT-PCR detection method and antigen-captured ELISA detection system were evaluated through specificity tests and sensitiveness tests.
二、四种BTV检测体系对BTV-5全病毒检测的评价对蓝舌病病病毒核酸实时荧光RT-PCR检测体系、核酸RT-PCR检测体系、OIE标准巢式RT-PCR检测方法和抗原捕获ELISA检测体系进行特异性和灵敏度评价。
参考来源 - 蓝舌病病毒四种检测技术比较研究·2,447,543篇论文数据，部分数据来源于NoteExpress
So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.
因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。
Conclusions The nested RT-PCR may be a rapid, accurate and efficient way for assisting early diagnosis of patients with SARS.
结论本研究建立的套式rt - PCR方法可作为发病早期SARS病人的辅助诊断。
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
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