METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
Method the canine multiplex system were used for PCR reaction and the product were analyzed by ABI310 genetic analyzer.
方法用建立的犬复合扩增体系进行PCR反应，用abi310型遗传分析仪对扩增产物进行检测。
The multiplex PCR is a progressing rapid detection method developed from traditional PCR detection technique.
多重PCR方法是建立在传统PCR检测技术基础上的快速简便的检测手段。
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