This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.

本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。

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This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.

本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。

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