Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.
利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 + T细胞，流式细胞术检测MACS分选前后CD 4 + T细胞比例，监测细胞分选效果。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Methods The survival rate and apoptosis rate of QBC939 after being treated with different density of 5-aza-2'-deoxycytidine were detected by using the method of MTT and flow cytometry.
方法应用MTT法检测不同浓度的5-氮-2’-脱氧胞苷对胆管癌细胞株QBC939存活率的影响，应用流式细胞术检测5-氮-2’-脱氧胞苷对QBC939细胞生长周期及凋亡率的影响。
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