Conclusion: ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.

结论：在复苏后早期阶段ES细胞培养中，不宜单独应用BRL条件培养基，须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层。

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Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.

目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。

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