The cells were counted and evaluated the survival rate with Live-Dead kit S-100 protein immunostaining was used to assesse the Schwann cell purity.

得到的细胞用台酚蓝染色计数细胞数和存活率，S - 100蛋白染色鉴定许旺细胞纯度。

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It is a cell-free filtrate vaccine, which means it contains no dead or live bacteria in the preparation.

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