孔板,制成固相抗体,往包被单抗的微孔中依次加入乙脑抗原(JEV-Ag),再与HRP 标记的 抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB 显色。
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RESULTS Short peptide displayed on phage specifically was bound to 2F2 and the binding was inhibited by natural JEV Ag.
结果筛选到的噬菌体能特异地与2 F2结合,并且这种结合可被天然抗原所抑制。
The peptide displayed on positive phage could react specifically with the mouse antiserum against natural JEV Ag .
阳性噬菌体展示肽也能与抗天然 JEV抗原的鼠血清产生特异性反应。
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