Objective:Analyzing the affecting factors of forming competent cells of Escherichia coli JM109 to increase transformation efficiency.

目的：分析大肠杆菌JM109感受态形成因素，提高转化效率。

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Of the six sectors needing transformation to increase efficiency and reduce oil use -- cars, trucks, planes, fuels, finance and military -- three or four are near or past their tipping point and starting to get easier.

CNN: Freeing America from its addiction to oil