Methods: PCO model of rat was established. Ovaries were fixed and processed for ultrastructural observation. DNA fragments in apoptotic cells were detected by in situ DNA 3 end labeling method.
方法：以大鼠多囊卵巢为动物模型，采用电子显微镜及3-末端原位标记法观察了大鼠多囊卵巢颗粒细胞凋亡的情况。
In situ end-labeling method (POD) indicates:HL-60 cells apoptosis induced by Liu-Shen-Wan alcohol extraction have time-concentration dependency relation within certain ranges;
原位末端标记表明：六神丸乙醇提取剂诱导HL-60细胞凋亡在一定范围内呈时间-浓度依赖关系；
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