Mouse uterine cervical cancer cell line U14 was used as the antigen to immunize mice.
以小鼠宫颈癌细胞系u14为抗原免疫小鼠。
The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
Methods: the mice were immunize with LOVO, and the mAb were screened by indirect cell ELISA and immunohistochemistry.
方法：用人结肠癌细胞株lovo免疫小鼠，用间接细胞ELISA和免疫组化的方法筛选抗结肠癌相关抗原的单抗。
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