...潮霉素; 转化子选择 [ 中图分类号 ] Q 943. 1 [ 文献标识码 ] A [gap=1481]Key words: A rabid op sis tha liana; su sp en sion cells; callu s; hyg rom ycin; selection of tran sfo rm an ts ..
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Regeneration of precocious trifoliate orange was completely suppressed when hygromycin B was used as selection agent. In recovery shoots, 9 months after grafting, 36.4% of the plantlets flowered (8/22).
实验中还发现,潮霉素对早实枳茎段的再生有强烈的抑制作用。
参考来源 - 柑橘高效遗传转化体系的建立与类胡萝卜素代谢相关基因的遗传转化The pooled plasmids were introduced into Arabidopsis upon Agrobacterium tumefciens-mediated transformation by Floral-dip methods. About 500 individual Hygromycin-B resistant plants were obtained from 100.000 seeds.
将过量表达文库的混合质粒经农杆菌介导用花序浸泡法进行拟南芥的遗传转化，在筛选的100.000粒种子中获得500株潮霉素抗性苗，3株表现出特异表型。
参考来源 - 碱蓬GST基因cDNA的克隆与表达及碱蓬cDNA过量表达文库转化拟南芥的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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