METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Special histochemical staining was used to detect HP in 166 biopsy specimens of duodenum and gastric antrum and for morphological observation.
方法：应用特殊染色方法对166例胃窦及十二指肠活检标本进行了形态学观察及HP检测。
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