As the former three substrates were employed, at high substrate concentration, L-Phe inhibited the enzyme uncompetitively and activated the enzyme at low substrate concentration.
在前三种底物条件下，当高底物浓度时，L-苯丙氨酸表现出反竞争性抑制作用； 低底物浓度时，则呈现激活作用。
In the batch reaction with high ratio of substrate to catalysts, the rate? Controlling step was determined by measuring the concentration variation of produced L-alanine in solution.
在近似无限浴的条件下，通过测量间歇拆分反应产生的L -丙氨酸的浓度变化规律，判断反应过程中的速率控制步骤。
But when concentration of CTX is high to some extent, CLBP can't combine with substrate because of competition effect, thus the synergic effect disappeared.
然而，一旦致死毒素达到一定浓度时，由于竞争效应，CLBP亲和力较低，无法与底物有效结合，从而导致协同作用效应消失。
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