The mean life of filter in observation group was shorter than that in control group(P<0.05).

观察组滤器的平均寿命短于对照组（P<0.05）。

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To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.

对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。

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