COI genes of hookworm was amplified from hookworm genomic DNA by PCR and sequenced. Program MEGA was used for the sequence analysis.

采集安徽和四川两省的十二指肠钩虫，用PCR技术扩增COI基因，同时测序并用MEGA软件进行遗传差异的分析。

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This efficient method is suitable for DNA sequence analysis, for the preparation of ssDNA probes, and potentially useful in studies of genomic structural organization.

此法不仅适用于疾病研究中的DNA测序，还可制各单链DNA探针，更利于基因结构组成的研究。

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