滋养细胞层（feeder cells layer）的制备 把长好的小鼠胚胎成纤维细胞的培养基吸掉，加入10ml新鲜的MEF培养基，并在避光的条件下加入110μl配好的丝裂霉素C，混匀后...
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Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken es in an undifferentiated state.
为维持细胞在培养过程中的未分化状态，需要采用饲养层细胞培养，同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。
Objective Adopt two methods to produce cells of feeder layer and compare and observe its result of culture to find a better method of culture.
目的采用两种方法制备饲养层细胞，对比观察培养效果，求得最佳培养方法，以期实现其有效利用。
AIM: Feeder layer cells are the necessary condition to prevent the differentiation of embryonic stem cells cultured in vitro.
目的：为保持胚胎干细胞的高度未分化状态，饲养层细胞成为胚胎干细胞体外培养中不可缺少的条件。
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