Dot hybridizations of RNA extracted from human lymphocytes were done by using the probe.
利用该探针与淋巴细胞RNA进行点杂交。
METHODS Total RNA was extracted from human embryonic lung fibroblast cells (WI 38) and the whole length gene of human OPG was obtained by RT PCR.
方法从培养的人胚肺成纤维细胞(WI - 38)中提取总rna，经rt - PCR获得人破骨细胞抑制因子基因。
Methods: DCs were derived from cord blood monocytes, then were pulsed by tumor associated antigen (TAA) extracted from human stomach cancer S-180 cell line.
方法：采用脐带血来源的单核细胞制备树突状细胞，体外经s - 180细胞肿瘤相关抗原(TAA)致敏后免疫小鼠，一周后接种s - 180细胞株。
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