Semi-quantitative PCR should be carried out at exponential phase. Cycles between 25 and 29 were appropriate for both adiponectin and 3 -actin.
半定量PCR要求PCR扩增在指数期进行,所以先要摸索指数期循环数,发现在25~29个循环脂联素和β-actin均处于指数期。
参考来源 - 猪脂联素基因的染色体定位、克隆、原核表达、抗血清制备及体内表达调控研究·2,447,543篇论文数据,部分数据来源于NoteExpress
The marine lysozyme has influences on the growth and metabolization of tested strains and the inhibition occurs at the beginning of the exponential phase of growth.
海洋微生物溶菌酶对试验菌株的生长繁殖和代谢均有影响,抑制作用主要发生在指数期初期。
According to their characteristics, the antagonistic substances produced by PNB in exponential phase were bacteriocin and they had been classified into small heat-stable peptide (SHSP).
根据其抑菌蛋白的性质,初步判断紫色非硫光合细菌在对数期产生的拮抗物质为细菌素,可归为小分子热稳定肽类。
Only a few commercially important enzymes are produced during exponential growth, the majority being synthesized during the post-exponential growth phase.
只有少数重要的商业酶是在指数生长期生产的,大多数酶是在指数生长期之后合成的。
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