Methods: Use indirect competitive ELISA method to detect kanamycin, and verify the method's recovery, linearity, specificity, precision, durability.

方法：采用间接竞争ELISA方法检测卡那霉素，并对方法的回收率、线性、特异性、精密性、耐用性等进行验证。

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Conclusion To detect HBV markers in serum, TRFIA offers many benefits in sensitivity, specificity, accuracy and practicability, and may be a routine method instead of MEIA and ELISA.

结论TRFIA技术检测HBV血清标志物灵敏度高、特异性强，可替代MEIA和ELISA而成为一种常规检测方法。

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ELISA-Crystal violet method was used to detect survival cells.

结晶紫方法测定A值，计算细胞存活量；

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