Besides the low efficiency of cloning - just 1.7 per cent of embryos came to term - another challenge to creating transgenic dogs is controlling where in the nuclear DNA a foreign gene lands.
除了低克隆率-只有1.7%的胚胎能存活到最后阶段-创造转基因狗还有一个挑战即如何控制外部基因在核d NA中的植入位置。
Donor cell selection is critical for the success of cloning animal, and it is also an important factor affecting the cloning efficiency.
但动物克隆效率仍然很低，供体细胞的选择是动物克隆中的重要步骤。
But, the efficency of cloning of PCR product is very poor because of the insufficiently cutting of two points of PCR fragment due to the low cutting efficiency of PCR product.
但是，由于PCR产物的酶切效率很低，致使片段两端不能够被完全切开，最终使得PCR产物的克隆效率较低。
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