METHODS: Immunofluorescence histochemical double-staining method was used.

方法：免疫荧光组织化学双重标记技术。

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FDA-PI double staining method is a new method to measure the algae cell viability.

PI双色荧光法为藻细胞活性的测定提供了新的方法。

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Methods: The staining method and washing step were reformed and tap-water was replaced by double distilled water.

方法：实验从染色冲洗的环节进行改良，采用蒸馏水(纯净水)取代自来水。

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