...： 正 本文以人低分化鼻咽癌(NPC)上皮细胞系(CNE-2Z)为对象,用蛋白激酶C(PKC)三种主要亚型的单克隆抗体,采用点印迹(Dot Blotting)法检测细胞颗粒部分与胞浆部分PKC各亚型的含量及分布.采用MTT
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3．斑点杂交 斑点杂交（dot blotting）即狭缝杂交，其 基本原理将被检样品点到一张硝酸纤维素膜上，烘烤固定， 预杂交后，经中和、洗脱、干燥，再将其和探针放入复性缓...
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斑点印迹杂交（dot blotting）和狭线印迹杂交 斑点印迹杂交（dot blotting）和狭线印迹杂交 （slot blotting ）是在Southern印迹杂交的基础上 ）是在Southern印...
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southern dot blotting southern点杂交
total rna dot blotting 总rna点杂交
reverse northern dot-blotting 反向northern斑点杂交
reverse dot blotting 反向点杂交
dot-blotting 斑点印迹法
dna dot blotting dna点杂交
rna dot blotting rna斑点杂交
dot blotting technique 斑点杂交技术
384 positive clones were randomly picked from each library and used for dot blotting. 50 significant differentially expression clones were sequenced and analyzed using BLAST.
从两个文库随机挑选384个白斑克隆进行斑点杂交进一步筛选,对其中差异显著的50个克隆进行测序分析。
参考来源 - 刺参(Apostichopus japonicus)高温胁迫消减cDNA文库的构建与分析The differentialy expressed clones screened by PCR from the forward cDNA library of microsporum canis incubated on mineral medium with child’s scalp tissue(tester) and adult’s scalp (driver2)were screened secondly by dot blotting.8.
7.利用斑点杂交方法对tester和driver2正向消减文库初步筛选出的部分阳性克隆进行第二轮筛选。
参考来源 - 利用SSH对犬小孢子菌在不同诱导底物表达差异基因的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
SDS-PAGE was used to detect the purity of target protein. A higher bioactivity of purified products was shown by the determination of dot-blotting assay and fibrinogen-clotting analysis.
通过SDS - PAGE检测融合蛋白的纯度，采用点杂交和纤维蛋白凝结活性检测分析，显示纯化产物具有较高生物活性。
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