MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法设计相应的引物,用RT _ PCR,基因克隆,DNA序列分析技术。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
DNA sequencing analysis proved that the sequence ofthe radiation-inducible promoter was totally consistent with the designing sequence.
合成启动子序列分析与设计序列完全一致;
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