dna polymerase gene pola dna多聚酶i基因
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
酚-氯仿法从外周血中提取基因组dna,聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法:采用多重聚合酶链反应方法,以提取的基因组d NA为模板,在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
Through DNA polymerase reaction, a modified lipase gene was obtained.
通过DNA聚合酶反应,获得了改造的荧光假单孢菌脂肪酶基因。
应用推荐