Conclusion: The DNA subtractive library may provide a solid foundation for screening and cloning pathogenic genes of UPEC132.
结论：所构建的基因组d NA消减文库为进一步筛选UPEC132中与致病相关基因奠定了基础。
Methods in metagenomic study, including DNA extraction, library construction and screening were discussed in this paper.
着重论述了宏基因组学的研究方法，包括DNA的提取、文库的构建以及筛选策略的选择。
Progress on the research of metagenomic DNA purification, metagenomic library construction and screening of soil microorganisms are reviewed briefly.
对土壤宏基因组DNA的提取、宏基因组文库的构建和筛选等方面的研究现状和进展进行了简要综述。
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