Method of making epileptic model Rats was fixed on stereotaxic apparatus and take the rhombus slices from the skull center so that cranium, anterior fontanelle and posterior fontanelle was exposured .
脑电描记方法 将大鼠头部固定在立体定位仪上,身体用胶带固定。 取头颅正中纵向切口,暴露颅骨及前卤、后卤。
参考来源 - 由CX32和CX43组成的缝隙连接在癫痫发病中的作用及调控机制的实验研究Methods The osteoblasts from newborn rat cranium were cultured in the presence of estradiol(1×10-10 mol/L,1×10-8 mol/L,1×10-6 mol/L) for 24 hours. Nitrophenol phosphate salt method and MTT assay were used to measure the alkaline phosphatase(ALP) and the cell proliferation of osteoblasts.
方法应用1×10-10mol/L、1×10-8mol/L、1×10-6mol/L浓度的雌二醇(estradiol,E2)分别作用原代培养的新生大鼠颅盖骨成骨细胞24h;采用MTT法和对硝基酚磷酸盐法检测成骨细胞增殖能力和细胞中碱性磷酸酶(ALP)活性;应用实时定量PCR和Western印迹杂交分析成骨细胞中IGF-2的mRNA和蛋白质的表达规律。
参考来源 - 雌激素对成骨细胞增殖及IGF·2,447,543篇论文数据,部分数据来源于NoteExpress
N-COUNT Your cranium is the round part of your skull that contains your brain. 颅 [技术]
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