Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.

结论采用无饲养层细胞培养体系构建自体角膜上皮组织，移植后可以成功修复角膜缘干细胞缺失的角膜上皮。

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Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.

目的采用无饲养层细胞培养体系构建家兔角膜上皮组织，自体移植后观察其对角膜缘干细胞缺失的治疗效果。

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The proliferation and refreshment of corneal epithelium depends on corneal limbal stem cells. Corneal limbal stem cell deficiency can lead to visual disorder.

角膜上皮的增生与更新依赖于角膜缘干细胞，角膜缘干细胞缺乏可导致视力障碍。

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