...Marine actinomyces;genetic transformation system;PEG;protoplast;conjugal transfer;PKS; 海洋放线菌;遗传转化体系;聚乙二醇;原生质体;接合转移;聚酮合成酶;
基于1个网页-相关网页
It is important to optimize the conditions of conjugative transfer for improving the efficiency of conjugal transfer. The results showed that high frequency exconjugants was obtained under the condition of 400μL mycelium of Micromonospora sp.
为了提高接合转移的频率,本研究对接合转移条件进行了优化。
参考来源 - 小单孢菌40027菌株位点特异性重组相关序列的克隆及分析To establish a gene transfer system in Streptomyces tenebrariu. s, a producer of apramycin, tobramycin and kanamycin B, several methods including PEG-mediated protoplasts transformation, conjugal transfer as well as electroporation were investigated.
本论文研究了安普霉素、妥布霉素和卡那霉素B的产生菌黑暗链霉菌的基因转移系统，探索了通过PEG-介导的原生质体转化、接合转移、电转化等方法向黑暗链霉菌中转入外源DNA的可能性。
参考来源 - 黑暗链霉菌基因转移系统的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
应用推荐