Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.

分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。

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There are encoding regions called exons and those are interrupted by non-coding regions called introns.

编码蛋白质的部分叫外显子,外显子被不编码蛋白质的内含子打断

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